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Anti-Cytokeratin 18 [RGE53]

AB03339-1.1
Absolute Antibody
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetKRT18
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Cytokeratin 18 [RGE53]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The antibody recognized glandular epithelial cells from female breast, digestive, respiratory, and urogenital tracts, endocrine and exocrine tissues, and mesothelial cells. Further, the antibody could distinguish adenocarcinomas and mesotheliomas, which stain positively in the indirect immunofluorescence technique (Ramaekers et al., 1983; PMID: 6193333). Further, the antibody produced fibrillar staining patterns in cultured HeLa cells, in cultured human hepatoma cells, F9 mouse teratocarcinoma cells and in a human colon carcinoma cell line (WiDr-218). The antibody detected Cytokeratin 18 in HeLa cells and cultured human hepatoma cells by western blot analysis (Ramaekers et al., 1983; PMID: 6350235). The antibody reacted with glandular and ductal epithelia, including those of the gastrointestinal tract, prostate, and kidney and with hepatocytes and mesothelium in immunohistochemistry (Ramaekers et al., 1990; PMID: 1690512).
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RGE53
  • Gene ID3875
  • Target name
    KRT18
  • Target description
    keratin 18
  • Target synonyms
    cell proliferation-inducing gene 46 protein; CK-18; CYK18; cytokeratin 18; K18; keratin 18, type I; keratin, type I cytoskeletal 18
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP05783
  • Protein Name
    Keratin, type I cytoskeletal 18
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 7. IHC analysis of Cytokeratin 18 using anti-Cytokeratin 18 antibody (A01357-1). Cytokeratin 18 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Cytokeratin 18 Antibody (A01357-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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