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Cytokeratin 19 antibody (MSVA-119M)

2273-119M
MS Validated
ApplicationsImmunoHistoChemistry
Product group Antibodies
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Overview

  • Supplier
    MS Validated
  • Product Name
    Cytokeratin 19 antibody (MSVA-119M)
  • Delivery Days Customer
    5
  • Applications
    ImmunoHistoChemistry
  • Applications Supplier
    IHC
  • Category Supplier
    Antibody
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MSVA-119M
  • Conjugate
    Unconjugated
  • Host
    Mouse
  • Isotype
    kappa, IgG
  • Protein IDP08727
  • Protein Name
    Keratin, type I cytoskeletal 19
  • Scientific Description
    Cytokeratin 19 antibody validated for immunohistochemistry on 76 different Normal Tissues
  • Reactivity Supplier
    Human
  • Storage Instruction
    Antibody with azide – store at 2 to 8 C. Antibody without azide – store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PB9715). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. Lane 2: human MCF-7 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human PANC-1 whole cell lysates, Lane 6: rat lung tissue lysates, Lane 7: rat small intestine tissue lysates, Lane 8: mouse lung tissue lysates, Lane 9: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 19 antigen affinity purified polyclonal antibody (Catalog # PB9715) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytokeratin 19 at approximately 44 kDa. The expected band size for Cytokeratin 19 is at 44 kDa.
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