Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 20(GTX110600) antibody at 1:200 dilution.
![Cytokeratin 20 antibody [N2C2], Internal detects Cytokeratin 20 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Cytokeratin 20 stained by Cytokeratin 20 antibody [N2C2], Internal (GTX110600) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX110600/GTX110600_45519_20250206_IHC-P_M_25022521_482.webp "Cytokeratin 20 antibody [N2C2], Internal detects Cytokeratin 20 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Cytokeratin 20 stained by Cytokeratin 20 antibody [N2C2], Internal (GTX110600) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 20 antibody [N2C2], Internal (GTX110600) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX110600/GTX110600_45393_20240426_WB_TPM_watermark_25092519_596.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 20 antibody [N2C2], Internal (GTX110600) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 20(GTX110600) antibody at 1:200 dilution.
Cytokeratin 20 antibody [N2C2], Internal
GTX110600
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetKRT20
Overview
- SupplierGeneTex
- Product NameCytokeratin 20 antibody [N2C2], Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.88 mg/ml
- ConjugateUnconjugated
- Gene ID54474
- Target nameKRT20
- Target descriptionkeratin 20
- Target synonymsCD20, CK-20, CK20, K20, KRT21, keratin, type I cytoskeletal 20, cytokeratin 20, keratin 20, type I, protein IT
- HostRabbit
- IsotypeIgG
- Protein IDP35900
- Protein NameKeratin, type I cytoskeletal 20
- Scientific DescriptionThe protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Fan Y, Huo X, Guo B, et al. Cullin 4b-RING ubiquitin ligase targets IRGM1 to regulate Wnt signaling and intestinal homeostasis. Cell Death Differ. 2022,29(9):1673-1688. doi: 10.1038/s41418-022-00954-9Read this paper
- Yen YT, Chien M, Lai YC, et al. PP2A Deficiency Enhances Carcinogenesis of Lgr5(+) Intestinal Stem Cells Both in Organoids and In Vivo. Cells. 2019,9(1). doi: 10.3390/cells9010090Read this paper
- Rajcevic U, Knol JC, Piersma S, et al. Colorectal cancer derived organotypic spheroids maintain essential tissue characteristics but adapt their metabolism in culture. Proteome Sci. 2014,12:39. doi: 10.1186/1477-5956-12-39Read this paper
Datasheet
Related products
Product group Antibodies
Anti-Cytokeratin 20 [10F6B12A9]AB03189-1.1-BT
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetKRT20
- SizePrice
Product group Antibodies
Anti-KRT20 AntibodyAMAB91564
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetKRT20
- SizePrice
Product group Antibodies
KRT20 Polyclonal AntibodyCAC15779
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetKRT20
- SizePrice
Product group Antibodies
CK20 Recombinant AntibodyBSM-52060R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Rat
TargetKRT20
- SizePrice
Product group Antibodies
KRT20 AntibodyCSB-PA002048
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetKRT20
- SizePrice
Product group Antibodies
Cytokeratin 20 antibody [ZM42]GTX01664
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetKRT20
- SizePrice
Product group Antibodies
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetKRT20
- SizePrice