CytoSelect Cell Proliferation Assay Reagent (Colorimetric)

Cell Biolabs

CytoSelect Cell Proliferation Assay Reagent (Colorimetric)

4

Reagent

Research Use Only

Cell Biolabs’ CytoSelect WST-1 Cell Proliferation Assay Reagent provides a colorimetric format for measuring and monitoring cell proliferation.  The 10 mL volume is sufficient for the evaluation of 960 assays in ten 96-well plates or 192 assays in eight 24-well plates.  Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then detected with the proliferation reagent, which is converted in live cells from WST-1 to the formazan form in the presence of cellular NADH and an electron mediator. An increase in cell proliferation is accompanied by increased signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells.

CytoSelect Cell Proliferation Assay Reagent (Colorimetric) is a clear, slightly red, ready-to-use solution and should be stored at 4°C. If precipitates or turbidity are observed upon thawing, warm the solution to 37°C for 5–10 minutes and agitate to dissolve the precipitates. For longer periods, aliquot and store at –20°C protected from light to avoid repeated freeze-thaw cycles.

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