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DAB Chromogen/HRP Substrate kit

Research Use Only
GTX73338
GeneTex
Product group Assays
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Overview

  • Supplier
    GeneTex
  • Product Name
    DAB Chromogen/HRP Substrate kit
  • Delivery Days Customer
    9
  • Application Supplier Note
    Components: 1) 5mL concentrated amber-colored DAB Chromogen Solution. 2) 200mL clear Stable DAB/Plus Substrate Buffer. 3) One empty mixing dropper bottle. Working Solution: Aliquot 1mL of Stable DAB/Plus Buffer in mixing bottle. Add 20microL (one drop) of concentrated Stable DAB/Plus Chromogen. Replace tip and mix. Testing Procedure: 1.) After peroxidase incubation, wash tissue sections with wash buffer. 2.) Wipe slides removing excess buffer. Add enough drops of working Stable DAB/Plus solution to cover tissue sections. 3.) Incubate for 5-10 minutes at room temperature. For optimal results, observe reaction under the microscope for signal development. Once the desired signal to noise ratio is achieved, stop the reaction by washing slides in buffer. Note: The working Stable DAB/Plus solution is stable for at least 2 weeks and should be prepared in an opaque bottle. Store at 2-8C when not in use. Any solution not used after this period should be discarded.
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Formulation
    Liquid
  • Scientific Description
    Peroxidase from the antibody detection system reacts with H2O2 substrate to degrade it,which then reacts with DAB, precipitating it at positive sites yielding a dark brown color.
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116133

References

  • The effect of the area proportion of the metastatic lesion within the central metastatic lymph node on response to therapy in papillary thyroid carcinoma. Shi L et al., 2021 Apr, Oncol Lett
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