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The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using CSB-PA337435(DAB2 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using CSB-PA337435(DAB2 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using CSB-PA337435(DAB2 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200)

DAB2 Antibody

CSB-PA337435
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetDAB2
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Overview

  • Supplier
    Cusabio
  • Product Name
    DAB2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1601
  • Target name
    DAB2
  • Target description
    DAB adaptor protein 2
  • Target synonyms
    adaptor molecule disabled-2; Dab, mitogen-responsive phosphoprotein, homolog 2; DAB2, clathrin adaptor protein; differentially expressed in ovarian carcinoma 2; differentially-expressed protein 2; disabled homolog 2; disabled homolog 2, mitogen-responsive phosphoprotein; DOC2; DOC-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP98082
  • Protein Name
    Disabled homolog 2
  • Scientific Description
    This gene encodes a mitogen-responsive phosphoprotein. It is expressed in normal ovarian epithelial cells, but is down-regulated or absent from ovarian carcinoma cell lines, suggesting its role as a tumor suppressor. This protein binds to the SH3 domains of GRB2, an adaptor protein that couples tyrosine kinase receptors to SOS (a guanine nucleotide exchange factor for Ras), via its C-terminal proline-rich sequences, and may thus modulate growth factor/Ras pathways by competing with SOS for binding to GRB2. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DAB2 using anti-DAB2 antibody (A01546-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat NRK whole cell lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAB2 antigen affinity purified polyclonal antibody (Catalog # A01546-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DAB2 at approximately 95 kDa. The expected band size for DAB2 is at 95 kDa.
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