DAF-2

Chemodex

DAF-2 [205391-01-1]

10

205391-01-1

Research Use Only

>95%

Warning

C20H14N2O5

362.34

Chemical. CAS: 205391-01-1. Formula: C20H14N2O5. MW: 362.34. Synthetic. DAF-2 is highly sensitive reagent for NO detection and determination of nitric oxide synthase activity. DAF-2, however, remains essentially nonfluorescent until it reacts with the nitrosonium cation (produced by spontaneous oxidation of nitric oxide) to form a fluorescent heterocycle, which becomes trapped in the cells cytoplasm. This sensitive fluorescent probe has been used to identify individual nitric oxide-producing neurons in brain slices, in mitochondria and in living plant cells. Spectral data: lambdaex 505nm; lambdaem 518nm in 0.1M Tris pH8.0. - DAF-2 is highly sensitive reagent for NO detection and determination of nitric oxide synthase activity. DAF-2, however, remains essentially nonfluorescent until it reacts with the nitrosonium cation (produced by spontaneous oxidation of nitric oxide) to form a fluorescent heterocycle, which becomes trapped in the cells cytoplasm. This sensitive fluorescent probe has been used to identify individual nitric oxide-producing neurons in brain slices, in mitochondria and in living plant cells. Spectral data: lambdaex 505nm; lambdaem 518nm in 0.1M Tris pH8.0.

NC1=CC2=C(C=C1N)C1(OC2=O)C2=CC=C(O)C=C2OC2=C1C=CC(O)=C2

-20°C,2°C to 8°C

41116134