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WB analysis of rat kidney tissue lysate using GTX53998 DAO antibody. Dilution : 1:3000 Loading : 25microg per lane
WB analysis of rat kidney tissue lysate using GTX53998 DAO antibody. Dilution : 1:3000 Loading : 25microg per lane
WB analysis of rat kidney tissue lysate using GTX53998 DAO antibody. Dilution : 1:3000 Loading : 25microg per lane

DAO antibody

GTX53998
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetDAO
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Overview

  • Supplier
    GeneTex
  • Product Name
    DAO antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1610
  • Target name
    DAO
  • Target description
    D-amino acid oxidase
  • Target synonyms
    DAAO; D-amino-acid oxidase; DAMOX; OXDA
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14920
  • Protein Name
    D-amino-acid oxidase
  • Scientific Description
    This gene encodes the peroxisomal enzyme D-amino acid oxidase. The enzyme is a flavoprotein which uses flavin adenine dinucleotide (FAD) as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. Its biological function is not known; it may act as a detoxifying agent which removes D-amino acids that accumulate during aging. In mice, it degrades D-serine, a co-agonist of the NMDA receptor. This gene may play a role in the pathophysiology of schizophrenia. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • D-Cysteine Activates Chaperone-Mediated Autophagy in Cerebellar Purkinje Cells via the Generation of Hydrogen Sulfide and Nrf2 Activation. Ueda E et al., 2022 Apr 5, Cells
    Read more
  • Therapeutic potential of d-cysteine against in vitro and in vivo models of spinocerebellar ataxia. Ohta T et al., 2021 Sep, Exp Neurol
    Read more

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Figure 1. Western blot analysis of DAO using anti-DAO antibody (A00773-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAO antigen affinity purified polyclonal antibody (Catalog # A00773-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DAO at approximately 39 kDa. The expected band size for DAO is at 39 kDa.
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