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DAP Antibody Pair

CSB-EAP01034
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityCanine, Hamster, Human, Porcine, Rabbit
TargetDAP
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Overview

  • Supplier
    Cusabio
  • Product Name
    DAP Antibody Pair
  • Delivery Days Customer
    9
  • Application Supplier Note
    We recommend using the capture antibody at a concentration of 0.8ug/ml and the detection antibody at a concentration of 0.4ug/ml.Optimal dilutions should be determined experimentally by the researcher.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Conjugate
    Biotin
  • Gene ID1611
  • Target name
    DAP
  • Target description
    death associated protein
  • Target synonyms
    DAP1, death-associated protein 1, DAP-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51397
  • Protein Name
    Death-associated protein 1
  • Reactivity
    Canine, Hamster, Human, Porcine, Rabbit
  • UNSPSC
    41116163

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Figure 1. Western blot analysis of DAP1/DAP using anti-DAP1/DAP antibody (A02756-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-937 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human U20S whole cell lysates, Lane 7: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAP1/DAP antigen affinity purified polyclonal antibody (Catalog # A02756-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DAP1/DAP at approximately 15 kDa. The expected band size for DAP1/DAP is at 15 kDa.
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