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Western blot All lanes: DARS2 antibody at 10ug/ml + MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 74 kDa Observed band size: 74 kDa
Western blot All lanes: DARS2 antibody at 10ug/ml + MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 74 kDa Observed band size: 74 kDa
Western blot All lanes: DARS2 antibody at 10ug/ml + MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 74 kDa Observed band size: 74 kDa

DARS2 Antibody

CSB-PA747490LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetDARS2
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Overview

  • Supplier
    Cusabio
  • Product Name
    DARS2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55157
  • Target name
    DARS2
  • Target description
    aspartyl-tRNA synthetase 2, mitochondrial
  • Target synonyms
    aspartate tRNA ligase 2, mitochondrial; aspartate--tRNA ligase, mitochondrial; aspartyl-tRNA synthetase, mitochondrial; ASPRS; LBSL; mtAspRS; MT-ASPRS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6PI48
  • Protein Name
    Aspartate--tRNA ligase, mitochondrial
  • Scientific Description
    mitochondrial matrix, mitochondrion, nucleus, aspartate-tRNA ligase activity, aspartate-tRNA(Asn) ligase activity, ATP binding, protein homodimerization activity, tRNA binding, mitochondrial asparaginyl-tRNA aminoacylation, tRNA aminoacylation
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DARS2 using anti-DARS2 antibody (A06034-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human U2OS whole cell lysates, Lane 6: human Caco-2 whole cell lysates, Lane 7: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DARS2 antigen affinity purified polyclonal antibody (Catalog # A06034-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DARS2 at approximately 74KD. The expected band size for DARS2 is at 74KD.
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