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Western Blot Positive WB detected in: Jurkat whole cell lysate All lanes: DAZAP1 antibody at 2.8microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44, 41 kDa Observed band size: 44, 70 kDa
Western Blot Positive WB detected in: Jurkat whole cell lysate All lanes: DAZAP1 antibody at 2.8microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44, 41 kDa Observed band size: 44, 70 kDa
Western Blot Positive WB detected in: Jurkat whole cell lysate All lanes: DAZAP1 antibody at 2.8microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44, 41 kDa Observed band size: 44, 70 kDa

DAZAP1 Antibody

CSB-PA822205LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetDAZAP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    DAZAP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID26528
  • Target name
    DAZAP1
  • Target description
    DAZ associated protein 1
  • Target synonyms
    DAZ-associated protein 1; deleted in azoospermia-associated protein 1; testicular tissue protein Li 50
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96EP5
  • Protein Name
    DAZ-associated protein 1
  • Scientific Description
    RNA-binding protein, which may be required during spermatogenesis.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DAZAP1 using anti-DAZAP1 antibody (A07947-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human AGS whole cell lysates, Lane 2: human GES-1 whole cell lysates, Lane 3: human HGC-27 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human 293T whole cell lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAZAP1 antigen affinity purified polyclonal antibody (Catalog # A07947-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DAZAP1 at approximately 43 kDa. The expected band size for DAZAP1 is at 43 kDa.
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