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DAZL Recombinant Antibody, Biotin Conjugated

BSM-61548R-BIOTIN
Bioss
ApplicationsWestern Blot
Product group Antibodies
TargetDAZL
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Overview

  • Supplier
    Bioss
  • Product Name
    DAZL Recombinant Antibody, Biotin Conjugated
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB(1:300-5000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Biotin
  • Gene ID1618
  • Target name
    DAZL
  • Target description
    deleted in azoospermia like
  • Target synonyms
    DAZH, DAZL1, DAZLA, SPGYLA, deleted in azoospermia-like, DAZ homolog, DAZ-like autosomal, SPGY-like-autosomal, deleted in azoospermia-like 1, germline specific RNA binding protein, spermatogenesis gene on the Y-like autosomal, testis secretory sperm-binding protein Li 204a
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92904
  • Protein Name
    Deleted in azoospermia-like
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DAZL using anti-DAZL antibody (A02069-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat testicular tissue lysates, Lane 3: mouse testicular tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAZL antigen affinity purified polyclonal antibody (Catalog # A02069-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DAZL at approximately 38KD. The expected band size for DAZL is at 33KD.
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