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Western Blot analysis of Jurkat cells using DBH Polyclonal Antibody
Western Blot analysis of Jurkat cells using DBH Polyclonal Antibody
Western Blot analysis of Jurkat cells using DBH Polyclonal Antibody

DBH Antibody

CSB-PA007654
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetDBH
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Overview

  • Supplier
    Cusabio
  • Product Name
    DBH Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1621
  • Target name
    DBH
  • Target description
    dopamine beta-hydroxylase
  • Target synonyms
    DBM, ORTHYP1, dopamine beta-hydroxylase, dopamine beta-hydroxylase (dopamine beta-monooxygenase)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09172
  • Protein Name
    Dopamine beta-hydroxylase
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DBH using anti-DBH antibody (A01110). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DBH antigen affinity purified polyclonal antibody (Catalog # A01110) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. A specific band was detected for DBH at approximately 75 kDa. The expected band size for DBH is at 70 kDa.
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