Inhibition of binding of DCAF1 complex to BDY DCAF1 Substrate by the inhibitor DCAF1 binder 1.
Inhibition of binding of DCAF1 complex to BDY DCAF1 Substrate by the inhibitor DCAF1 binder 1.
DCAF1 Binding Assay Kit
82519
Product group Assays
Overview
- SupplierBPS Bioscience
- Product NameDCAF1 Binding Assay Kit
- Delivery Days Customer7
- CertificationResearch Use Only
- Scientific DescriptionThe DCAF1 Binding Assay Kit is a fluorescence polarization (FP), homogeneous, 96-well assay kit designed for the screening and profiling of molecules that inhibit the binding of the DCAF1 (DDB1 and CUL4 Associated Factor 1) Complex to a high affinity DCAF1 fluorescent probe. The kit contains enough purified recombinant DCAF1/Rbx1 (M5I)/DDB1/CUL4A Complex, fluorescent probe and assay buffer for 100 reactions. The assay uses a DCAF1 fluorescent probe. This is a very small molecule that rotates fast (low FP). This probe is incubated with the DCAF1 complex leading to the formation of a large complex, with restricted movement (high FP). FP is proportional to binding of DCAF1 to the fluorescent probe. This assay requires a fluorescent microplate reader capable of measuring fluorescence polarization (FP) and equipped with the required parts to read the FP signal. For more information FP technology, visit our Tech Note: FP, assay principles and applications.
- Storage InstructionSee manual
- UNSPSC41116133