DCAF5 Intrachain TR-FRET Assay Kit

BPS Bioscience

DCAF5 Intrachain TR-FRET Assay Kit

23

Research Use Only

DDB1- and CUL4-associated factor 5

The DCAF5 Intrachain TR-FRET Assay Kit is a homogeneous, sensitive TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) assay kit designed to measure DCAF5 (DDB1- and CUL4-associated factor 5) complex auto-ubiquitination activity. It utilizes a mix of donor and acceptor labeled ubiquitin to complete the TR-FRET pairing. This assay measures poly-ubiquitination since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains formed on DCAF5, making it suitable for real-time kinetics analyses of poly-ubiquitination. The kit contains enough purified DCAF5 complex, purified UBE1 and UbcH5b, detection reagents and assay buffer for 400 reactions.

41116100

Human