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Chemical Structure
Chemical Structure
Chemical Structure

DCFDA [2044-85-1]

Research Use Only
CDX-D0063
Chemodex
CAS Number2044-85-1
Product group Chemicals
Estimated Purity>95%
Molecular Weight485.27
Price on request
Packing Size
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Overview

  • Supplier
    Chemodex
  • Product Name
    DCFDA [2044-85-1]
  • Delivery Days Customer
    10
  • CAS Number
    2044-85-1
  • Certification
    Research Use Only
  • Estimated Purity
    >95%
  • Hazard Information
    Non-hazardous
  • Molecular Formula
    C24H14Cl2O7
  • Molecular Weight
    485.27
  • Scientific Description
    A cell-permeable fluorogenic dye useful for the detection of reactive oxygen species (ROS) and nitric oxide (NO) and for the determination of the degree of overall oxidative stress. The fluorescence of DCFDA indicates oxidation by hydrogen peroxide, peroxynitrite or hydroxyl radical. Superoxide anions can also contribute to DCFDA oxidation albeit at a lesser degree. It measures hydroxyl, peroxyl and other reactive oxygen species (ROS) activity within the cell. After diffusion in to the cell, DCFDA is deacetylated by cellular esterases to a non-fluorescent compound, which is later oxidized by ROS into the highly fluorescent 2,7-dichlorofluorescein (DCF). Spectral data: lambdaex=504nm, lambdaem=524nm in 0.1 M Tris pH 8.0 (esterase). - Chemical. CAS: 2044-85-1. Formula: C24H14Cl2O7. MW: 485.27. Synthetic. A cell-permeable fluorogenic dye useful for the detection of reactive oxygen species (ROS) and nitric oxide (NO) and for the determination of the degree of overall oxidative stress. The fluorescence of DCFDA indicates oxidation by hydrogen peroxide, peroxynitrite or hydroxyl radical. Superoxide anions can also contribute to DCFDA oxidation albeit at a lesser degree. It measures hydroxyl, peroxyl and other reactive oxygen species (ROS) activity within the cell. After diffusion in to the cell, DCFDA is deacetylated by cellular esterases to a non-fluorescent compound, which is later oxidized by ROS into the highly fluorescent 2,7-dichlorofluorescein (DCF). Spectral data: lambdaex=504nm, lambdaem=524nm in 0.1 M Tris pH 8.0 (esterase).
  • SMILES
    CC(=O)OC1=C(Cl)C=C2C(OC3=CC(OC(C)=O)=C(Cl)C=C3C22OC(=O)C3=C2C=CC=C3)=C1
  • Storage Instruction
    2°C to 8°C,RT
  • UNSPSC
    41116134