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Western Blot analysis of Jurkat HuvEc cells using SMIF Polyclonal Antibody
Western Blot analysis of Jurkat HuvEc cells using SMIF Polyclonal Antibody
Western Blot analysis of Jurkat HuvEc cells using SMIF Polyclonal Antibody

DCP1A Antibody

CSB-PA004119
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetDCP1A
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Overview

  • Supplier
    Cusabio
  • Product Name
    DCP1A Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55802
  • Target name
    DCP1A
  • Target description
    decapping mRNA 1A
  • Target synonyms
    HSA275986, Nbla00360, SMAD4IP1, SMIF, mRNA-decapping enzyme 1A, DCP1 decapping enzyme homolog A, DCP1 decapping enzyme-like protein A, Smad4-interacting transcriptional co-activator, decapping enzyme hDcp1a, putative protein product of Nbla00360, transcription factor SMIF
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NPI6
  • Protein Name
    mRNA-decapping enzyme 1A
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DCP1A using anti-DCP1A antibody (A04587-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCP1A antigen affinity purified polyclonal antibody (Catalog # A04587-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DCP1A at approximately 75 kDa. The expected band size for DCP1A is at 75 kDa.
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