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Western Blot analysis of Hela cells using TRP2 Polyclonal Antibody
Western Blot analysis of Hela cells using TRP2 Polyclonal Antibody
Western Blot analysis of Hela cells using TRP2 Polyclonal Antibody

DCT Antibody

CSB-PA004336
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetDCT
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Overview

  • Supplier
    Cusabio
  • Product Name
    DCT Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1638
  • Target name
    DCT
  • Target description
    dopachrome tautomerase
  • Target synonyms
    dopachrome delta-isomerase; dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2); DT; L-dopachrome Delta-isomerase; L-dopachrome isomerase; L-dopachrome tautomerase; OCA8; TRP2; TRP-2; tyrosinase related protein-2; tyrosine-related protein 2; TYRP2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP40126
  • Protein Name
    L-dopachrome tautomerase
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TRP2/DCT using anti-TRP2/DCT antibody (A01830-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRP2/DCT antigen affinity purified polyclonal antibody (Catalog # A01830-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRP2/DCT at approximately 59 kDa. The expected band size for TRP2/DCT is at 59 kDa.
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