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DCTPP1 / XTP3TPA Antibody

LS-C830894
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetDCTPP1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    DCTPP1 / XTP3TPA Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:5000 - 1:10000), IHC (1:100 - 1:300)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID79077
  • Target name
    DCTPP1
  • Target description
    dCTP pyrophosphatase 1
  • Target synonyms
    CDA03, RS21C6, XTP3TPA, dCTP pyrophosphatase 1, XTP3-transactivated gene A protein, XTP3-transactivated protein A, dCTPase 1, deoxycytidine-triphosphatase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of XTP3TPA/DCTPP1 using anti-XTP3TPA/DCTPP1 antibody (A11334-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XTP3TPA/DCTPP1 antigen affinity purified polyclonal antibody (Catalog # A11334-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XTP3TPA/DCTPP1 at approximately 19 kDa. The expected band size for XTP3TPA/DCTPP1 is at 19 kDa.
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ReactivityHuman, Mouse
TargetDCTPP1
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TargetDCTPP1
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