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Western Blot analysis of HepG2 cells using DDX3 Polyclonal Antibody
Western Blot analysis of HepG2 cells using DDX3 Polyclonal Antibody
Western Blot analysis of HepG2 cells using DDX3 Polyclonal Antibody

DDX3X Antibody

CSB-PA002106
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetDDX3X
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Overview

  • Supplier
    Cusabio
  • Product Name
    DDX3X Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1654
  • Target name
    DDX3X
  • Target description
    DEAD-box helicase 3 X-linked
  • Target synonyms
    CAP-Rf, DBX, DDX14, DDX3, HLP2, MRX102, MRXSSB, ATP-dependent RNA helicase DDX3X, DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked, DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked, DEAD box protein 3, X-chromosomal, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3, DEAD/H box-3, helicase-like protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00571
  • Protein Name
    ATP-dependent RNA helicase DDX3X
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DDX3 using anti-DDX3 antibody (A00751-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HT1080 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX3 antigen affinity purified polyclonal antibody (Catalog # A00751-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDX3 at approximately 73 kDa. The expected band size for DDX3 is at 73 kDa.
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