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DDX6 antibody

GTX102795
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetDDX6
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Overview

  • Supplier
    GeneTex
  • Product Name
    DDX6 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.71 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1656
  • Target name
    DDX6
  • Target description
    DEAD-box helicase 6
  • Target synonyms
    ATP-dependent RNA helicase p54; DEAD (Asp-Glu-Ala-Asp) box helicase 6; DEAD (Asp-Glu-Ala-Asp) box polypeptide 6; DEAD box protein 6; DEAD box-6; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD); HLR2; IDDILF; oncogene RCK; P54; probable ATP-dependent RNA helicase DDX6; RCK; Rck/p54
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP26196
  • Protein Name
    Probable ATP-dependent RNA helicase DDX6
  • Scientific Description
    This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Sequestration of LINE-1 in cytosolic aggregates by MOV10 restricts retrotransposition. Arora R et al., 2022 Sep 5, EMBO Rep
    Read more
  • RNA decay in processing bodies is indispensable for adipogenesis. Maeda R et al., 2021 Mar 17, Cell Death Dis
    Read more
  • EDC3 phosphorylation regulates growth and invasion through controlling P-body formation and dynamics. Bearss JJ et al., 2021 Apr 7, EMBO Rep
    Read more
  • Imsnc761 and DDX6 synergistically suppress cell proliferation and promote apoptosis via p53 in testicular embryonal carcinoma cells. Duan Z et al., 2018 Aug 31, Biosci Rep
    Read more
  • Resources for the Comprehensive Discovery of Functional RNA Elements. Sundararaman B et al., 2016 Mar 17, Mol Cell
    Read more

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Figure 1. Western blot analysis of DDX6 using anti-DDX6 antibody (A03826-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human Raji whole cell lysates, Lane 6: human THP-1 whole cell lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates, Lane 9: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX6 antigen affinity purified polyclonal antibody (Catalog # A03826-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDX6 at approximately 54KD. The expected band size for DDX6 is at 54KD.
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