DEAC-dUTP
ORB178360
Estimated Purity≥95%
Product group Chemicals
Molecular WeightTheoretical MW: 766.48 g/mol (free acid); Detected MW: 766.11 g/mol (free acid)
Overview
- SupplierBiorbyt
- Product NameDEAC-dUTP
- Delivery Days Customer10
- Application Supplier NoteIncorporation into DNA/cDNA by Nick Translation with DNAse I/DNA Polymerase Iin-house data. Spectroscopic Propertie: lambdaexc 426 nm, lambdaem 480 nm, epsilon 57.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).
- CertificationResearch Use Only
- Concentration1.0-1.1 mM
- Estimated Purity≥95%
- Molecular WeightTheoretical MW: 766.48 g/mol (free acid); Detected MW: 766.11 g/mol (free acid)
- Scientific DescriptionDEAC-dUTP is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substi-tute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended DEAC-dUTP/dTTP ratio for Nick Translation: 30-50% DEAC-dUTP/70-50% dTTP.
- Storage Instruction-20°C
- UNSPSC12352200