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FACS analysis of mouse peripheral blood granulocytes using GTX42986 Dectin-1 antibody [2A11] (PE).
FACS analysis of mouse peripheral blood granulocytes using GTX42986 Dectin-1 antibody [2A11] (PE).
FACS analysis of mouse peripheral blood granulocytes using GTX42986 Dectin-1 antibody [2A11] (PE).

Dectin-1 antibody [2A11] (PE)

GTX42986
GeneTex
ApplicationsFlow Cytometry
Product group Antibodies
TargetClec7a
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Overview

  • Supplier
    GeneTex
  • Product Name
    Dectin-1 antibody [2A11] (PE)
  • Delivery Days Customer
    9
  • Application Supplier Note
    FACS: Neat-1/10. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2A11
  • Conjugate
    RPE
  • Gene ID56644
  • Target name
    Clec7a
  • Target description
    C-type lectin domain family 7, member a
  • Target synonyms
    beta-; beta-glucan receptor; beta-GR; BG; BGR; Clecsf; Clecsf12; C-type (calcium dependent, carbohydrate recognition domain) lectin, superfamily member 12; C-type lectin domain family 7 member A; C-type lectin superfamily member 12; DC-associated C-type lectin 1; dectin; dectin-1; dendritic cell-associated C-type lectin 1
  • Host
    Rat
  • Isotype
    IgG2b
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • Novel probiotic yeast from Miso promotes regulatory dendritic cell IL-10 production and attenuates DSS-induced colitis in mice. Okada Y et al., 2021 Sep, J Gastroenterol
    Read more
  • The receptor DNGR-1 signals for phagosomal rupture to promote cross-presentation of dead-cell-associated antigens. Canton J et al., 2021 Feb, Nat Immunol
    Read more
  • Characteristics of fucose-containing polysaccharides from submerged fermentation of Agaricus blazei Murill.
    Read more
  • Immune Enhancing Activity of beta-(1,3)-Glucan Isolated from Genus Agrobacterium in Bone-Marrow Derived Macrophages and Mice Splenocytes. Byun EB et al., 2016, Am J Chin Med
    Read more
  • Recognition of Salmonella by Dectin-1 induces presentation of peptide antigen to type B T cells. Jackson N et al., 2014 Apr, Eur J Immunol
    Read more
  • A Type II Arabinogalactan from Anoectochilus formosanus for G-CSF Production in Macrophages and Leukopenia Improvement in CT26-Bearing Mice Treated with 5-Fluorouracil. Yang LC et al., 2013, Evid Based Complement Alternat Med
    Read more
  • Antisense oligonucleotide treatment enhances the recovery of acute lung injury through IL-10-secreting M2-like macrophage-induced expansion of CD4+ regulatory T cells. Guo Z et al., 2013 Apr 15, J Immunol
    Read more

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Figure 1. Western blot analysis of Dectin-1/Clec7a using anti-Dectin-1/Clec7a antibody (A02731-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dectin-1/Clec7a antigen affinity purified polyclonal antibody (Catalog # A02731-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dectin-1/Clec7a at approximately 33 kDa. The expected band size for Dectin-1/Clec7a is at 33 kDa.
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