Non-infected (–) and infected (+) BHK-21 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus NS4A protein antibody (GTX132069) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Dengue virus NS4A protein antibody detects Dengue virus NS4A protein protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded BHK-21 infected with dengue virus. Green: Dengue virus NS4A protein stained by Dengue virus NS4A protein antibody (GTX132069) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX132069/GTX132069_42466_20181026_IHC-P-FL_virus_w_23060523_681.webp "Dengue virus NS4A protein antibody detects Dengue virus NS4A protein protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded BHK-21 infected with dengue virus. Green: Dengue virus NS4A protein stained by Dengue virus NS4A protein antibody (GTX132069) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
Non-infected (–) and infected (+) BHK-21 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus NS4A protein antibody (GTX132069) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Dengue virus NS4A protein antibody
GTX132069
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityVirus
Overview
- SupplierGeneTex
- Product NameDengue virus NS4A protein antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.12 mg/ml
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- ReactivityVirus
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Richardson RB, Ohlson MB, Eitson JL, et al. A CRISPR screen identifies IFI6 as an ER-resident interferon effector that blocks flavivirus replication. Nat Microbiol. 2018,3(11):1214-1223. doi: 10.1038/s41564-018-0244-1Read this paper