Dengue virus NS5 protein antibody [GT361] detects Dengue virus 2 NS5 protein at nucleus by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in paraformaldehyde. Green: NS5 (Dengue virus 2) protein stained by Dengue virus NS5 protein antibody [GT361] (GTX629447) diluted at 1:2000. Blue: Hoechst 33342 staining.
![Non-infected (–) and infected (+) C6/36 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus NS5 protein antibody [GT361] (GTX629447) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX629447/GTX629447_44503_20211119_WB_Denguevirus2_w_23061202_554.webp "Non-infected (–) and infected (+) C6/36 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus NS5 protein antibody [GT361] (GTX629447) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.")
![Dengue virus NS5 protein antibody [GT361] detects Dengue virus 2 NS5 protein by western blot analysis. A. 30 μg BHK21 whole cell lysate/extract B. 30 μg whole cell lysate/extract of Dengue virus 2 infected BHK21 cells C. 30 μg whole cell lysate/extract of Japanese encephalitis virus infected BHK21 cells 10% SDS-PAGE Dengue virus NS5 protein antibody [GT361] (GTX629447) dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX629447/GTX629447_41358_WB_DV2_w_23061202_260.webp "Dengue virus NS5 protein antibody [GT361] detects Dengue virus 2 NS5 protein by western blot analysis. A. 30 μg BHK21 whole cell lysate/extract B. 30 μg whole cell lysate/extract of Dengue virus 2 infected BHK21 cells C. 30 μg whole cell lysate/extract of Japanese encephalitis virus infected BHK21 cells 10% SDS-PAGE Dengue virus NS5 protein antibody [GT361] (GTX629447) dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.")
![Dengue virus NS5 protein antibody [GT361] detects Dengue virus 2 NS5 protein protein at nucleus by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in paraformaldehyde. Green: NS5 (Dengue virus 2) protein stained by Dengue virus NS5 protein antibody [GT361] (GTX629447) diluted at 1:2000. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX629447/GTX629447_41358_IFA_w_23061202_816.webp "Dengue virus NS5 protein antibody [GT361] detects Dengue virus 2 NS5 protein protein at nucleus by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in paraformaldehyde. Green: NS5 (Dengue virus 2) protein stained by Dengue virus NS5 protein antibody [GT361] (GTX629447) diluted at 1:2000. Blue: Hoechst 33342 staining.")
![Dengue virus NS5 protein antibody [GT361] detects Dengue virus 2 NS5 protein by western blot analysis. A. 30 μg BHK-21 whole cell extract B. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells C. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells 7.5% SDS-PAGE Dengue virus NS5 protein antibody [GT361] (GTX629447) dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX629447/GTX629447_41358_WB_multiple-Dengue-virus_w_23061202_924.webp "Dengue virus NS5 protein antibody [GT361] detects Dengue virus 2 NS5 protein by western blot analysis. A. 30 μg BHK-21 whole cell extract B. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells C. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells 7.5% SDS-PAGE Dengue virus NS5 protein antibody [GT361] (GTX629447) dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.")
![Non-infected (–) and infected (+) BHK-21 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS5 protein antibody [GT361] (GTX629447) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX629447/GTX629447_44503_20230804_WB_multiplevirus_23080901_831.webp "Non-infected (–) and infected (+) BHK-21 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS5 protein antibody [GT361] (GTX629447) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.")
Dengue virus NS5 protein antibody [GT361] detects Dengue virus 2 NS5 protein at nucleus by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in paraformaldehyde. Green: NS5 (Dengue virus 2) protein stained by Dengue virus NS5 protein antibody [GT361] (GTX629447) diluted at 1:2000. Blue: Hoechst 33342 staining.
Dengue virus NS5 protein antibody [GT361]
GTX629447
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityVirus
Overview
- SupplierGeneTex
- Product NameDengue virus NS5 protein antibody [GT361]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. ICC/IF: 1:100-1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT361
- Concentration1 mg/ml
- ConjugateUnconjugated
- HostMouse
- IsotypeIgG1
- ReactivityVirus
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Butler M, Chotiwan N, Brewster CD, et al. Cyclin-Dependent Kinases 8 and 19 Regulate Host Cell Metabolism during Dengue Virus Serotype 2 Infection. Viruses. 2020,12(6). doi: 10.3390/v12060654Read this paper
- Xie X, Zou J, Zhang X, et al. Dengue NS2A Protein Orchestrates Virus Assembly. Cell Host Microbe. 2019,26(5):606-622.e8. doi: 10.1016/j.chom.2019.09.015Read this paper
- Ngo AM, Shurtleff MJ, Popova KD, et al. The ER membrane protein complex is required to ensure correct topology and stable expression of flavivirus polyproteins. Elife. 2019,8. doi: 10.7554/eLife.48469Read this paper
- Lim SP, Noble CG, Seh CC, et al. Potent Allosteric Dengue Virus NS5 Polymerase Inhibitors: Mechanism of Action and Resistance Profiling. PLoS Pathog. 2016,12(8):e1005737. doi: 10.1371/journal.ppat.1005737Read this paper
- Alves RPDS, Pereira LR, Fabris DLN, et al. Production of a Recombinant Dengue Virus 2 NS5 Protein and Potential Use as a Vaccine Antigen. Clin Vaccine Immunol. 2016,23(6):460-469. doi: 10.1128/CVI.00081-16Read this paper