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Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with \tDEPTOR Polyclonal Antibody, Unconjugated (bs-8255R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with \tDEPTOR Polyclonal Antibody, Unconjugated (bs-8255R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with \tDEPTOR Polyclonal Antibody, Unconjugated (bs-8255R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.

DEPTOR Polyclonal Antibody

BS-8255R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetDEPTOR
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Overview

  • Supplier
    Bioss
  • Product Name
    DEPTOR Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID64798
  • Target name
    DEPTOR
  • Target description
    DEP domain containing MTOR interacting protein
  • Target synonyms
    DEP.6, DEPDC6, hDEPTOR, DEP domain-containing mTOR-interacting protein, DEP domain containing 6, DEP domain-containing protein 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TB45
  • Protein Name
    DEP domain-containing mTOR-interacting protein
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Liu X, Cai S, Zhang C, et al. Deacetylation of NAT10 by Sirt1 promotes the transition from rRNA biogenesis to autophagy upon energy stress. Nucleic Acids Res. 2018,46(18):9601-9616. doi: 10.1093/nar/gky777
    Read this paper

Datasheet

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