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ProductsBack/Desmoplakin Recombinant Antbody Kit (KD-Validated)

Desmoplakin Recombinant Antbody Kit (KD-Validated)

BSM-90225R
Bioss Antibodies
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetDSP
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Desmoplakin Recombinant Antbody Kit (KD-Validated)
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IC(1:1000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID1832
  • Target name
    DSP
  • Target description
    desmoplakin
  • Target synonyms
    250/210 kDa paraneoplastic pemphigus antigen; DCWHKTA; desmoplakin; DP
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15924
  • Protein Name
    Desmoplakin
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Desmoplakin using anti-Desmoplakin antibody (A00616-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Desmoplakin antigen affinity purified polyclonal antibody (Catalog # A00616-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Desmoplakin at approximately 250-280KD. The expected band size for Desmoplakin is at 332KD.
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