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ProductsBack/DFFA Antibody - N-terminal region (ARP30359_T100)

DFFA Antibody - N-terminal region (ARP30359_T100)

ARP30359_T100
Aviva Systems Biology
ApplicationsWestern Blot
Product group Antibodies
TargetDFFA
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    DFFA Antibody - N-terminal region (ARP30359_T100)
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Format
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
  • Gene ID1676
  • Target name
    DFFA
  • Target description
    DNA fragmentation factor subunit alpha
  • Target synonyms
    DFF-45, DFF1, ICAD, DNA fragmentation factor subunit alpha, DFF45, DNA fragmentation factor 45 kDa subunit, DNA fragmentation factor, 45kDa, alpha polypeptide, inhibitor of CAD
  • Host
    Rabbit
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DFFA/ICAD using anti-DFFA/ICAD antibody (A03671-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human HEPG2 whole cell lysates, Lane 4: rat stomach tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DFFA/ICAD antigen affinity purified polyclonal antibody (Catalog # A03671-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DFFA/ICAD at approximately 45KD. The expected band size for DFFA/ICAD is at 45KD.
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