Paraformaldehyde-fixed, paraffin embedded rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with DFNA5 Polyclonal Antibody, Unconjugated (bs-14286R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with DFNA5 Polyclonal Antibody, Unconjugated (bs-14286R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
DFNA5 Polyclonal Antibody
BS-14286R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGSDME
Overview
- SupplierBioss
- Product NameDFNA5 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID1687
- Target nameGSDME
- Target descriptiongasdermin E
- Target synonymsDFNA5, ICERE-1, gasdermin-E, DFNA5, deafness associated tumor suppressor, inversely correlated with estrogen receptor expression 1, non-syndromic hearing impairment protein 5, nonsyndromic hearing impairment protein
- HostRabbit
- IsotypeIgG
- Protein IDO60443
- Protein NameGasdermin-E
- Storage Instruction-20°C
- UNSPSC12352203
Datasheet
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