DGCR8 Recombinant Monoclonal Antibody
CSB-RA845175A0HU
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetDGCR8
Overview
- SupplierCusabio
- Product NameDGCR8 Recombinant Monoclonal Antibody
- Delivery Days Customer20
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID2H2
- ConjugateUnconjugated
- Gene ID54487
- Target nameDGCR8
- Target descriptionDGCR8 microprocessor complex subunit
- Target synonymsC22orf12; DGCRK6; DiGeorge syndrome critical region 8; DiGeorge syndrome critical region gene 8; Gy1; microprocessor complex subunit DGCR8; pasha
- IsotypeIgG
- Protein IDQ8WYQ5
- Protein NameMicroprocessor complex subunit DGCR8
- Scientific DescriptionComponent of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs (PubMed:26027739, PubMed:26748718). The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding (PubMed:15531877, PubMed:15574589, PubMed:15589161, PubMed:16751099, PubMed:16906129, PubMed:16963499, PubMed:17159994). Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing (PubMed:25799998). Involved in the silencing of embryonic stem cell self-renewal (By similarity).
- ReactivityHuman
- Storage Instruction-20°C or -80°C
- UNSPSC12352203