DIG-11-dUTP

Jena Bioscience

DIG-11-dUTP

5

Incorporation into DNA/cDNA byPCR with Taq polymerase[1], in-house dataNick Translation with DNAse I/ DNA Polymerase I[2]Primer Extension with Klenow exo- [3]3-End Labeling with Terminal deoxynucleotidyl Transferase (TdT)[4]Reverse Transcription with MMLV Reverse Transcriptase[2]

Aminoallyl-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR, Nick Translation, Primer Extension, 3-End Labeling and Reverse Transcription. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Amine-functionalized DNA/cDNA can subsequently be labeled via the classic Amine/NHS Ester reaction that offers the choice to introduce a Biotin group (via NHS Ester of Biotin) for subsequent purification tasksto introduce fluorescent group (via NHS Ester of fluorescent dyes) for subsequent microscopic imaging

2°C to 8°C,RT

41106305