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ProductsBack/Dishevelled 3 antibody [N1N2], N-term

Dishevelled 3 antibody [N1N2], N-term

GTX102509
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Zebra Fish
TargetDVL3
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Overview

  • Supplier
    GeneTex
  • Product Name
    Dishevelled 3 antibody [N1N2], N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.99 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1857
  • Target name
    DVL3
  • Target description
    dishevelled segment polarity protein 3
  • Target synonyms
    DRS3, segment polarity protein dishevelled homolog DVL-3, dishevelled 3 (homologous to Drosophila dsh), dishevelled, dsh homolog 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92997
  • Protein Name
    Segment polarity protein dishevelled homolog DVL-3
  • Scientific Description
    This gene is a member of a multi-gene family which shares strong similarity with the Drosophila dishevelled gene, dsh. The Drosophila dishevelled gene encodes a cytoplasmic phosphoprotein that regulates cell proliferation. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Zebra Fish
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Pai VC, Hsu CC, Chan TS, et al. ASPM promotes prostate cancer stemness and progression by augmenting Wnt-Dvl-3-β-catenin signaling. Oncogene. 2019,38(8):1340-1353. doi: 10.1038/s41388-018-0497-4
    Read this paper
  • Wang J, Qian J, Hoeksema MD, et al. Integrative genomics analysis identifies candidate drivers at 3q26-29 amplicon in squamous cell carcinoma of the lung. Clin Cancer Res. 2013,19(20):5580-90. doi: 10.1158/1078-0432.CCR-13-0594
    Read this paper
  • Rodriguez-Gil DJ, Hu W, Greer CA. Dishevelled proteins are associated with olfactory sensory neuron presynaptic terminals. PLoS One. 2013,8(2):e56561. doi: 10.1371/journal.pone.0056561
    Read this paper

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Figure 1. Western blot analysis of Dishevelled 3 using anti-Dishevelled 3 antibody (A03577). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dishevelled 3 antigen affinity purified polyclonal antibody (Catalog # A03577) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dishevelled 3 at approximately 78KD. The expected band size for Dishevelled 3 is at 78KD.
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