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Immunohistochemical staining of human fallopian tube shows moderate positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows moderate positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows moderate positivity in glandular cells.

Anti-DVL3 Antibody

HPA058265
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetDVL3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-DVL3 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1857
  • Target name
    DVL3
  • Target description
    dishevelled segment polarity protein 3
  • Target synonyms
    DRS3, segment polarity protein dishevelled homolog DVL-3, dishevelled 3 (homologous to Drosophila dsh), dishevelled, dsh homolog 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92997
  • Protein Name
    Segment polarity protein dishevelled homolog DVL-3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of Dishevelled 3 using anti-Dishevelled 3 antibody (A03577). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dishevelled 3 antigen affinity purified polyclonal antibody (Catalog # A03577) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dishevelled 3 at approximately 78KD. The expected band size for Dishevelled 3 is at 78KD.
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