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DKK1 Antibody

LS-C343550
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetDKK1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    DKK1 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:80000), WB (1:1000 - 1:5000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID22943
  • Target name
    DKK1
  • Target description
    dickkopf WNT signaling pathway inhibitor 1
  • Target synonyms
    dickkopf 1 homolog; dickkopf-1 like; dickkopf-like protein 1; dickkopf-related protein 1; DKK-1; SK
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Image kindly provided by Dr. Magdalena Krol. Control tumor cells, tumor cells grown in macrophage-conditioned medium, tumor cells sorted from co-culture with macrophages, and macrophages from monocultures and sorted from co-culture with tumor cells were analyzed. Total protein concentrations in lysates were determined using a Bio-Rad protein assay. Proteins (50 mg) were resolved using SDS-PAGE and transferred onto PVDF membranes. The membranes were then blocked with 5% non-fat dry milk in TBS buffer containing 0.5% Tween 20. The membranes were then incubated overnight with the primary Rabbit Anti-DKK1 Polyclonal Antibody at 1:100 dilution. Subsequently, the membranes were washed three times in TBS containing 0.5% Tween 20 and incubated for 1 h at room temperature with secondary antibodies conjugated with the appropriate infrared (IR) fluorophore IRDyeH 800 CW or IRDyeH 680 RD at a dilution of 1:5000.
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