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Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with DLL1 antibody (GTX133745) diluted at 1:500 and competitor's antibody (sc-9102) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with DLL1 antibody (GTX133745) diluted at 1:500 and competitor's antibody (sc-9102) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with DLL1 antibody (GTX133745) diluted at 1:500 and competitor's antibody (sc-9102) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

DLL1 antibody

GTX133745
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetDLL1
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Overview

  • Supplier
    GeneTex
  • Product Name
    DLL1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.81 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID28514
  • Target name
    DLL1
  • Target description
    delta like canonical Notch ligand 1
  • Target synonyms
    DELTA1, DL1, Delta, NEDBAS, delta-like protein 1, H-Delta-1, drosophila Delta homolog 1, epididymis secretory sperm binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00548
  • Protein Name
    Delta-like protein 1
  • Scientific Description
    DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis. It may play a role in cell-to-cell communication. [provided by RefSeq]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of DLL1 using anti-DLL1 antibody (A02513-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: huamn Jurkat whole cell lysates, Lane 4: huamn Raji whole cell lysates, Lane 5: human SW620 whole cell lysates, Lane 6: human HepG2 whole cell lysates, Lane 7: human U20S whole cell lysates, Lane 8: rat heart tissue lysates, Lane 9: rat C6 whole cell lysates, Lane 10: mouse heart tissue lysates, Lane 11: mouse Neuro-2a whole cell lysates, Lane 12: mouse RAW264.7 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLL1 antigen affinity purified polyclonal antibody (Catalog # A02513-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DLL1 at approximately 78KD. The expected band size for DLL1 is at 78KD.
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