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Dmc1 (3C134)

SC-70965
Santa Cruz Biotechnology
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetDMC1
200 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    Dmc1 (3C134)
  • Delivery Days Customer
    5
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID11144
  • Target name
    DMC1
  • Target description
    DNA meiotic recombinase 1
  • Target synonyms
    DMC1H, LIM15, dJ199H16.1, meiotic recombination protein DMC1/LIM15 homolog, DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination, disrupted meiotic cDNA1, yeast, homolog of
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DMC1 using anti-DMC1 antibody (A02978-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DMC1 antigen affinity purified polyclonal antibody (Catalog # A02978-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DMC1 at approximately 37 kDa. The expected band size for DMC1 is at 37 kDa.
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