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ProductsBack/DMRT1 Polyclonal Antibody
Formalin-fixed and paraffin embedded mouse testis labeled with Anti-DMO/Dmrt1a Polyclonal Antibody, Unconjugated (bs-11276R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse testis labeled with Anti-DMO/Dmrt1a Polyclonal Antibody, Unconjugated (bs-11276R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse testis labeled with Anti-DMO/Dmrt1a Polyclonal Antibody, Unconjugated (bs-11276R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

DMRT1 Polyclonal Antibody

BS-11276R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetDMRT1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    DMO/Dmrt1a Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1761
  • Target name
    DMRT1
  • Target description
    doublesex and mab-3 related transcription factor 1
  • Target synonyms
    CT154; DM domain expressed in testis 1; DM domain expressed in testis protein 1; DMT1; doublesex- and mab-3-related transcription factor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y5R6
  • Protein Name
    Doublesex- and mab-3-related transcription factor 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Expression and cellular localization of double sex and mab-3 related transcription factor 1 in testes of postnatal Small-Tail Han sheep at different developmental stages. Li T et al., 2018 Feb 5, Gene
    Read more

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Figure 1. Western blot analysis of DMRT1 using anti-DMRT1 antibody (RP1083). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DMRT1 antigen affinity purified polyclonal antibody (Catalog # RP1083) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DMRT1 at approximately 39KD. The expected band size for DMRT1 is at 39KD.
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