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ProductsBack/DNA Damage Inducible Transcript 3 (DDIT3) Polyclonal Antibody

DNA Damage Inducible Transcript 3 (DDIT3) Polyclonal Antibody

CAU21757
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetDdit3
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Overview

  • Supplier
    Biomatik
  • Product Name
    DNA Damage Inducible Transcript 3 (DDIT3) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID13198
  • Target name
    Ddit3
  • Target description
    DNA-damage inducible transcript 3
  • Target synonyms
    AltDDIT3, CHOP-10, CHOP10, chop, gadd153, DNA damage-inducible transcript 3 protein, C/EBP homoologous protein 10, C/EBP zeta, CCAAT/enhancer-binding protein homologous protein, DDIT-3, alternative DDIT3 proteins, c/EBP-homologous protein 10, growth arrest and DNA-damage-inducible protein GADD153
  • Host
    Rabbit
  • Protein IDP35639
  • Protein Name
    DNA damage-inducible transcript 3 protein
  • Scientific Description
    The DNA Damage Inducible Transcript 3 (DDIT3) Polyclonal Antibody (Species: Mouse) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity
    Mouse, Rat
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Ddit3 using anti-Ddit3 antibody (A00311-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse RAW264.7 whole cell lysates, Lane 2: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ddit3 antigen affinity purified polyclonal antibody (Catalog # A00311-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ddit3 at approximately 29 kDa. The expected band size for Ddit3 is at 29 kDa.
Product group Antibodies
Boster Bio
Anti-Ddit3 Antibody Picoband(r)A00311-4-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityMouse, Rat
TargetDdit3
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