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ProductsBack/DNA Polymerase alpha Colorimetric Cell-Based ELISA

DNA Polymerase alpha Colorimetric Cell-Based ELISA

CBCAB00377
Assay Genie
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Overview

  • Supplier
    Assay Genie
  • Product Name
    DNA Polymerase alpha Colorimetric Cell-Based ELISA
  • Delivery Days Customer
    9
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Gene ID5422
  • Target name
    POLA1
  • Target description
    DNA polymerase alpha 1, catalytic subunit
  • Target synonyms
    DNA polymerase alpha catalytic subunit; DNA polymerase alpha catalytic subunit p180; NSX; p180; POLA; polymerase (DNA directed), alpha 1, catalytic subunit; polymerase (DNA) alpha 1, catalytic subunit; polymerase (DNA-directed), alpha (70kD); VEODS
  • Protein IDP09884
  • Protein Name
    DNA polymerase alpha catalytic subunit
  • Shelf life instruction
    6 months
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116158

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Figure 1. Western blot analysis of POLA1 using anti-POLA1 antibody (A04421-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLA1 antigen affinity purified polyclonal antibody (Catalog # A04421-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLA1 at approximately 180 kDa. The expected band size for POLA1 is at 180 kDa.
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