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DNAJC6 Antibody

PACO26273
Assay Genie
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetDNAJC6
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Overview

  • Supplier
    Assay Genie
  • Product Name
    DNAJC6 Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC, IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID9829
  • Target name
    DNAJC6
  • Target description
    DnaJ heat shock protein family (Hsp40) member C6
  • Target synonyms
    DJC6, PARK19, auxilin, DnaJ (Hsp40) homolog, subfamily B, member 6, DnaJ (Hsp40) homolog, subfamily C, member 6, putative tyrosine-protein phosphatase auxilin
  • Host
    Rabbit
  • Protein IDO75061
  • Protein Name
    Auxilin
  • Scientific Description
    DNAJC6 Antibody is a premium polyclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for ELISA, IHC, IF, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 50ug format to meet your experimental needs. For optimal performance, store at -20°C or -80°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of DNAJC6 using anti-DNAJC6 antibody (A05616-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNAJC6 antigen affinity purified polyclonal antibody (Catalog # A05616-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNAJC6 at approximately 120 kDa. The expected band size for DNAJC6 is at 100 kDa.
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