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ProductsBack/DNase II Polyclonal Antibody
Human colon cancer 4% Paraformaldehyde-fixed and paraffin-embedded.\nAntigen retrieval: Citrate buffer ( 0.01M, pH 6.0 ) boiling bath for 15min; Blocking buffer (normal goat serum 10%) at 37℃ for 20 min;\nIncubation: Anti-DNase II Polyclonal Antibody, Unconjugated(bs-7652R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue) was used to stain the cell nuclei\n
Human colon cancer 4% Paraformaldehyde-fixed and paraffin-embedded.\nAntigen retrieval: Citrate buffer ( 0.01M, pH 6.0 ) boiling bath for 15min; Blocking buffer (normal goat serum 10%) at 37℃ for 20 min;\nIncubation: Anti-DNase II Polyclonal Antibody, Unconjugated(bs-7652R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue) was used to stain the cell nuclei\n
Human colon cancer 4% Paraformaldehyde-fixed and paraffin-embedded.\nAntigen retrieval: Citrate buffer ( 0.01M, pH 6.0 ) boiling bath for 15min; Blocking buffer (normal goat serum 10%) at 37℃ for 20 min;\nIncubation: Anti-DNase II Polyclonal Antibody, Unconjugated(bs-7652R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue) was used to stain the cell nuclei\n

DNase II Polyclonal Antibody

BS-7652R
Bioss
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetDNASE2
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Overview

  • Supplier
    Bioss
  • Product Name
    DNase II Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), IHC-P(1:200-400), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1777
  • Target name
    DNASE2
  • Target description
    deoxyribonuclease 2, lysosomal
  • Target synonyms
    AIPCS, DNASE2A, DNL, DNL2, deoxyribonuclease-2-alpha, DNase II alpha, DNase II, lysosomal, R31240_2, acid DNase, deoxyribonuclease II alpha, deoxyribonuclease II, lysosomal, lysosomal DNase II
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00115
  • Protein Name
    Deoxyribonuclease-2-alpha
  • Reactivity
    Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Takahashi A, Loo TM, Okada R, et al. Downregulation of cytoplasmic DNases is implicated in cytoplasmic DNA accumulation and SASP in senescent cells. Nat Commun. 2018,9(1):1249. doi: 10.1038/s41467-018-03555-8
    Read this paper

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