DNase IL3 antibody [N3C3] detects DNase IL3 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNase IL3 stained by DNase IL3 antibody [N3C3] (GTX114363) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
![Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with DNase IL3 antibody [N3C3] (GTX114363) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX114363/GTX114363_44608_20220311_WB_22081423_860.webp "Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with DNase IL3 antibody [N3C3] (GTX114363) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DNase IL3 antibody [N3C3] (GTX114363) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX114363/GTX114363_44608_20220311_WB_M_tissue_22081423_286.webp "Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DNase IL3 antibody [N3C3] (GTX114363) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DNase IL3 antibody [N3C3] (GTX114363) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX114363/GTX114363_44608_20231222_WB_23122619_467.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DNase IL3 antibody [N3C3] (GTX114363) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
DNase IL3 antibody [N3C3] detects DNase IL3 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNase IL3 stained by DNase IL3 antibody [N3C3] (GTX114363) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
DNase IL3 antibody [N3C3]
GTX114363
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetDNASE1L3
Overview
- SupplierGeneTex
- Product NameDNase IL3 antibody [N3C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.93 mg/ml
- ConjugateUnconjugated
- Gene ID1776
- Target nameDNASE1L3
- Target descriptiondeoxyribonuclease 1L3
- Target synonymsD3, DHP2, DNAS1L3, LSD, SLEB16, deoxyribonuclease gamma, DNase I homolog protein 2, DNase I homolog protein DHP2, DNase I-like 3, DNase gamma, DNaseY, LS-DNase, Liver and spleen DNase, deoxyribonuclease 1 like 3, deoxyribonuclease I-like 3, deoxyribonuclease I-like III
- HostRabbit
- IsotypeIgG
- Protein IDQ13609
- Protein NameDeoxyribonuclease gamma
- Scientific DescriptionThis gene encodes a member of the DNase family. The protein hydrolyzes DNA, is not inhibited by actin, and mediates the breakdown of DNA during apoptosis. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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