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DNM3 / Dynamin 3 Antibody

LS-C400717
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetDNM3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    DNM3 / Dynamin 3 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID26052
  • Target name
    DNM3
  • Target description
    dynamin 3
  • Target synonyms
    Dyna III, dynamin-3, T-dynamin, dynamin family member, dynamin, testicular
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Dynamin 3/DNM3 using anti-Dynamin 3/DNM3 antibody (A06890-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dynamin 3/DNM3 antigen affinity purified polyclonal antibody (Catalog # A06890-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dynamin 3/DNM3 at approximately 110 kDa. The expected band size for Dynamin 3/DNM3 is at 98 kDa.
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