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WB analysis of A431 lysate using GTX89965 DOCK1 antibody, C-term. Dilution : 4microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of A431 lysate using GTX89965 DOCK1 antibody, C-term. Dilution : 4microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of A431 lysate using GTX89965 DOCK1 antibody, C-term. Dilution : 4microg/ml Loading : 35microg protein in RIPA buffer

DOCK1 antibody, C-term

GTX89965
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetDOCK1
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Overview

  • Supplier
    GeneTex
  • Product Name
    DOCK1 antibody, C-term
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 3-4microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1793
  • Target name
    DOCK1
  • Target description
    dedicator of cytokinesis 1
  • Target synonyms
    180 kDa protein downstream of CRK; ced5; dedicator of cytokinesis protein 1; DOCK180; DOwnstream of CrK
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDQ14185
  • Protein Name
    Dedicator of cytokinesis protein 1
  • Scientific Description
    This gene encodes a member of the dedicator of cytokinesis protein family. Dedicator of cytokinesis proteins act as guanine nucleotide exchange factors for small Rho family G proteins. The encoded protein regulates the small GTPase Rac, thereby influencing several biological processes, including phagocytosis and cell migration. Overexpression of this gene has also been associated with certain cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DOCK180/DOCK1 using anti-DOCK180/DOCK1 antibody (A03440-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DOCK180/DOCK1 antigen affinity purified polyclonal antibody (Catalog # A03440-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DOCK180/DOCK1 at approximately 215 kDa. The expected band size for DOCK180/DOCK1 is at 215 kDa.
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