Dog Cort (Corticosterone) ELISA Kit

Assay Genie

Dog Cort (Corticosterone) ELISA Kit

12

ELISA

1.57-100 ng/mL

0.51 ng/mL

Research Use Only

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog Cort. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog Cort. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dog Cort in the samples is then determined by comparing the OD of the samples to the standard curve.

2°C to 8°C

41116100