ELISA Results of Purified Goat Anti-Dog IgG F(c) Antibody tested against purified Dog IgG F(c). Each well was coated in duplicate with 10 µg of Dog IgG F(c) (p/n 004-0103) [Red Line] and Dog IgG (p/n 004-0102) [Green Line]. The working dilution of Dog IgG F(c) is 1:48,000. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer (p/n MB-066), Donkey Anti-Goat IgG mx8 HRP conjugated and TMB substrate (p/n TMBE-1000).