Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Unboiled SH-SY5Y whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)](https://www.genetex.com/upload/website/prouct_img/normal/GTX636952/GTX636952_44711_20220701_WB_Fraction_22070501_113.webp "Unboiled SH-SY5Y whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)")
![Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636952/GTX636952_44711_20221118_WB_22112219_534.webp "Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX636952/GTX636952_44711_20221209_ICC_IF_R_22122018_313.webp "Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
![Unboiled mouse tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636952/GTX636952_T-44662_20220527_WB_M_brain_competitor_watermark_w_23061202_146.webp "Unboiled mouse tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.")
![Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636952/GTX636952_T-44662_20220610_WB_brain_competitor_watermark_w_23061202_495.webp "Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.")
![Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:500 and competitor's antibody (Ab5084P) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636952/GTX636952_T-44662_20220610_WB_R_tissue_competitor_watermark_w_23061202_127.webp "Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:500 and competitor's antibody (Ab5084P) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.")
![Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:1000 and competitor's antibody (Ab5084p) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636952/GTX636952_T-44662_20220610_WB_M_tissue_competitor_watermark_w_23061202_121.webp "Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:1000 and competitor's antibody (Ab5084p) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.")
![Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein at cytoplasm by immunofluorescent analysis. Sample: SH-SY5Y cells were fixed in ice-cold MeOH for 5 min. Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636952/GTX636952_T-44662_20220429_ICC_IF_w_23061203_743.webp "Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein at cytoplasm by immunofluorescent analysis. Sample: SH-SY5Y cells were fixed in ice-cold MeOH for 5 min. Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.")
![Dopamine Receptor D2 antibody [HL1478] (GTX636952) specifically detects DRD2 protein on a VirD TM -GPCR array. Yellow: DRD2 White: DRD3 Blue: DRD5 Bottom left: Positive control](https://www.genetex.com/upload/website/prouct_img/normal/GTX636952/GTX636952_44711_20230815_GPCRarray_watermark_23081420_403.webp "Dopamine Receptor D2 antibody [HL1478] (GTX636952) specifically detects DRD2 protein on a VirD TM -GPCR array. Yellow: DRD2 White: DRD3 Blue: DRD5 Bottom left: Positive control")
Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1478] (GTX636952) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Dopamine Receptor D2 antibody [HL1478]
GTX636952
ApplicationsFunctional Assay, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetDRD2
Overview
- SupplierGeneTex
- Product NameDopamine Receptor D2 antibody [HL1478]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFunctional Assay, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1478
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID1813
- Target nameDRD2
- Target descriptiondopamine receptor D2
- Target synonymsD2DR, D2R, D(2) dopamine receptor, dopamine D2 receptor, seven transmembrane helix receptor
- HostRabbit
- IsotypeIgG
- Protein IDP14416
- Protein NameD(2) dopamine receptor
- Scientific DescriptionThis gene encodes the D2 subtype of the dopamine receptor. This G-protein coupled receptor inhibits adenylyl cyclase activity. A missense mutation in this gene causes myoclonus dystonia; other mutations have been associated with schizophrenia. Alternative splicing of this gene results in two transcript variants encoding different isoforms. A third variant has been described, but it has not been determined whether this form is normal or due to aberrant splicing. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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![Dopamine Receptor D2 antibody [HL1584] detects Dopamine Receptor D2 protein by immunofluorescent analysis. Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1584] (GTX637063) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX637063/GTX637063_44788_20221230_ICC_IF_22122901_234.webp)
Product group Antibodies
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Product group Antibodies
Dopamine Receptor D2 antibodyGTX71747
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