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Dopamine Transporter Antibody

HY-P81165
MedChem Express
TargetSLC6A3
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Dopamine Transporter
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID6531
  • Target name
    SLC6A3
  • Target description
    solute carrier family 6 member 3
  • Target synonyms
    DA transporter; DAT; DAT1; dopamine transporter 1; PKDYS; PKDYS1; sodium-dependent dopamine transporter; solute carrier family 6 (neurotransmitter transporter), member 3; solute carrier family 6 (neurotransmitter transporter, dopamine), member 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Dopamine Transporter Antibody is an unconjugated, approximately 68 kDa, rabbit-derived, anti-Dopamine Transporter polyclonal antibody. Dopamine Transporter Antibody can be used for: WB, ELISA, IHC-P, IHC-F, IF expriments in human, mouse, rat, and predicted: dog, cow background without labeling.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Dopamine Transporter/SLC6A3 using anti-Dopamine Transporter/SLC6A3 antibody (A00369-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dopamine Transporter/SLC6A3 antigen affinity purified polyclonal antibody (Catalog # A00369-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dopamine Transporter/SLC6A3 at approximately 68 kDa. The expected band size for Dopamine Transporter/SLC6A3 is at 68 kDa.
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