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DPH5 Antibody

LS-C816274
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetDPH5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    DPH5 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC-P (1:200 - 1:1000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID51611
  • Target name
    DPH5
  • Target description
    diphthamide biosynthesis 5
  • Target synonyms
    AD-018, CGI-30, HSPC143, NEDSFF, NPD015, diphthine methyl ester synthase, DPH5 homolog, diphthamide biosynthesis methyltransferase, diphthine synthase, protein x 0011
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DPH5 using anti-DPH5 antibody (A11989-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DPH5 antigen affinity purified polyclonal antibody (Catalog # A11989-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DPH5 at approximately 37 kDa. The expected band size for DPH5 is at 32 kDa.
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Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
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