DpnI

Applied Biological Materials

DpnI

9

Research Use Only

The DpnI restriction enzyme digests DNA at GmeATC sites, requiring N6-methylation of the adenine residue for activity. DNA purified from a dam+ E. coli strain will be a substrate for DpnI due to the adenine methylation. DpnI cleaves hemi-methylated dam sites 60X more slowly than fully methylated dam sites.

-20°C

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